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KMID : 0388120120210010029
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Journal of Korean Society for the Study of Obesity
2012 Volume.21 No. 1 p.29 ~ p.36
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The Effect of AMPK Activation on Wnt and sFRP5 in TNF-α Induced Adipocyte Metabolic Dysfunction in 3T3-L1 Cell
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Hong Seok-Woo
Lee Jin-Mi
Choi Ji-Hun
Park Se-Eun
Rhee Eun-Jung
Park Cheol-Young
Oh Ki-won
Park Sung-woo
Kim Sun-Woo
Lee Won-young
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Abstract
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Background: Chronic inflammation in adipose tissue is closely associated with metabolic diseases, such as, type 2 diabetes and obesity. In the present study, we investigated the Wnt signaling pathway and fat metabolism in the
course of TNF-α-induced inflammation and recovery in 3T3-L1 adipocyte.
Methods: We stimulated the fully differentiated 3T3-L1 adipocyte with either TNF-α only or with both TNF-α and
AICAR for 24 hrs to induce inflammation. We assessed the alteration of Wnt signaling pathway and the factors associated with fat metabolism with western blot assay and real-time PCR.
Results: The expression of inflammatory cytokines, IL-6 and MCP-1, was increased by TNF-α treatment in fully
differentiated 3T3-L1 adipocyte, and the marked activation of Wnt5a, a noncanonical Wnt ligand, was observed. The
expression of PPAR-γ was reduced, and the lipolysis measured by glycerol release, was markedly increased. The
activation of AMPK by AICAR inhibited the TNF-α-induced inflammation, reversed the alteration in Wnt signaling
pathway, and reversed fat metabolism induced by TNF-α. AMPK activation stimulated the secretion of sFRP5, an anti-inflammatory and anti-Wnt signaling adipokine.
Conclusion: The activation of AMPK suppressed noncanonical Wnt signaling pathway and protected the adipoctye from inflammation and lipolysis, induced by TNF-α treatment, by sFRP5 stimulation. Based on these results,
we suggest that noncanonical Wnt signaling pathway and sFRP may have important roles in metabolic diseases such
as, obesity and diabetes.
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KEYWORD
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Adipocyte, Inflammation, Noncanonical Wnt signaling pathway, sFRP5, AMPK, TNF-α
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